[题名] 全铁氟龙微流控芯片

[作者] Kangning Ren, Wen Dai, Jianhua Zhou, Jing Su, and Hongkai Wu

[期刊] 美国国家科学院院刊

[摘要] 尽管微流控技术展现出了令人振奋的潜力，但其广泛应用受到了用于制造它们的材料的缺点的严重限制。在本工作中，我们提出了一种方便的策略，用于制造具有集成阀门的全氟聚合物微流控芯片，这些阀门对各种化学物质具有出色的惰性，并且对所有溶剂具有极高的抵抗力。与其它微流控材料（例如聚二甲基硅氧烷（PDMS））相比，全氟聚合物芯片具有更多优点，例如不吸收小分子、很少将生物分子吸附到通道壁上、以及没有从材料主体中提取残余分子进入通道中的溶液。在全氟聚合物通道中培养了各种生物细胞。贴壁细胞可以附着在通道底部，扩散并良好地在通道中增殖（与具有相同尺寸的PDMS通道中的细胞增殖率相似）。全氟聚合物材料具有良好的透气性，使其适合长时间在微通道内培养细胞。

[关键词] 微流体、全氟聚合物、微芯片、芯片上细胞培养、溶剂电阻芯片

[Title] Whole-Teflon microfluidic chips

[Author] Kangning Ren, Wen Dai, Jianhua Zhou, Jing Su, and Hongkai Wu

[Journal] Proceedings of the National Academy of Sciences

[Abstract] Although microfluidics has shown exciting potential, its broad applications are significantly limited by drawbacks of the materials used to make them. In this work, we present a convenient strategy for fabricating whole-Teflon microfluidic chips with integrated valves that show outstanding inertness to various chemicals and extreme resistance against all solvents. Compared with other microfluidic materials [e.g., poly(dimethylsiloxane) (PDMS)] the whole-Teflon chip has a few more advantages, such as no absorption of small molecules, little adsorption of biomolecules onto channel walls, and no leaching of residue molecules from the material bulk into the solution in the channel. Various biological cells have been cultured in the whole-Teflon channel. Adherent cells can attach to the channel bottom, spread, and proliferate well in the channels (with similar proliferation rate to the cells in PDMS channels with the same dimensions). The moderately good gas permeability of the Teflon materials makes it suitable to culture cells inside the microchannels for a long time.

[Keywords] microfluidic、Teflon materials、microchips 、on-chip cell culture 、solvent resistive chip